nucleus pulposus histologic degeneration (collagen fiber destruction, vessel/nerve ingrowth) in indigo carmine-stained versus unstained disc tissue

increases, discolored (indigo carmine-stained) nucleus pulposus tissue showed collagen fiber destruction and vessel/nerve ingrowth consistent with degeneration, n = 5

Not recorded as a finding: this platform holds no node for the endpoint

However, in discolored part, slits with destruction of collagen fiber array and ingrowth of vessel and nerve were observed.

PMID:16100472 · one of two readings recorded this

nucleus pulposus histology in stained (discolored) vs unstained tissue

increases, discolored (stained) tissue showed collagen fiber destruction and vessel/nerve ingrowth, consistent with degeneration, versus normal architecture in undiscolored tissue, n = 5

However, in discolored part, slits with destruction of collagen fiber array and ingrowth of vessel and nerve were observed.

PMID:16100472 · one of two readings recorded this

human chondrocyte cytotoxicity assessed by light microscopy morphology, vital cell counting, and LDH expression after in vitro indigo carmine exposure

increases

Not recorded as a finding: this platform holds no node for the endpoint

LDH expression was significantly increased after treatment with 100% indigo carmine.

PMID:23341851 · one of two readings recorded this

human chondrocyte viability and cytotoxicity (vital cell count and lactate dehydrogenase, LDH, expression) following in vitro exposure to indigo carmine

increases

Not recorded as a finding: this platform holds no node for the endpoint

LDH expression was significantly increased after treatment with 100% indigo carmine.

PMID:23341851 · one of two readings recorded this

decolorization rate of indigo during ozonation, kinetic study

increases

The result of kinetic studies showed that the presence of CNTs-COOH dramatically increased the decolorization rate of indigo.

PMID:25462739 · one of two readings recorded this

Decolorization rate of indigo during ozonation

increases

The result of kinetic studies showed that the presence of CNTs-COOH dramatically increased the decolorization rate of indigo.

PMID:25462739 · one of two readings recorded this

Mineralization degree (total organic carbon, TOC) during ozonation

increases

The presence of the catalyst CNTs-COOH leads to a higher mineralization degree than single ozonation, as suggested by the total organic carbon (TOC) measurement.

PMID:25462739 · one of two readings recorded this

total organic carbon (TOC) mineralization degree

increases

The presence of the catalyst CNTs-COOH leads to a higher mineralization degree than single ozonation, as suggested by the total organic carbon (TOC) measurement.

PMID:25462739 · one of two readings recorded this

oxygen reserve index (ORi™) by pulse co-oximetry after intravenous indigo carmine injection

decreases

Not recorded as a finding: this platform holds no node for the endpoint

ORi rapidly decreased after indigo carmine intravenous injection in all patients.

PMID:28975476 · one of two readings recorded this

oxygen reserve index (ORi), measured by pulse co-oximetry, following intravenous indigo carmine injection during elective gynecologic surgery

decreases

Not recorded as a finding: this platform holds no node for the endpoint

ORi rapidly decreased after indigo carmine intravenous injection in all patients.

PMID:28975476 · one of two readings recorded this

oxygen reserve index (ORi) measured by pulse co-oximetry monitoring after intravenous indigo carmine injection during gynecologic surgery

decreases

Not recorded as a finding: this platform holds no node for the endpoint

ORi rapidly decreased after indigo carmine intravenous injection in all patients.

PMID:28975476 · one of two readings recorded this

oxygen reserve index (ORi™)

decreases, n = 19

ORi rapidly decreased after indigo carmine intravenous injection in all patients.

PMID:28975476 · one of two readings recorded this

complications related to MIL injection

no effect, no complications related to injected MIL

No complication related to the injected MIL occurred.

PMID:30819492 · one of two readings recorded this

hepatic mitochondrial State 3 oxygen consumption (ADP-stimulated respiration), by oxygen electrode (Hansatech-Oxygraph Plus)

decreases

Not recorded as a finding: this platform holds no node for the endpoint

The IRIC group worsened in State3 when compared to the IR group.

PMID:32996998 · one of two readings recorded this

Mitochondrial osmotic swelling (inner membrane permeability transition)

The mitochondrial osmotic swelling representing the transition of the mitochondrial internal membrane permeability, showed a significant difference between the SH x IR groups; SHIC x IRIC and SH x IRIC.

PMID:32996998 · one of two readings recorded this

mitochondrial State 3 (ADP-stimulated) oxygen consumption in isolated liver mitochondria, Hansatech Oxygraph Plus oxygen electrode

decreases

Not recorded as a finding: this platform holds no node for the endpoint

The IRIC group worsened in State3 when compared to the IR group.

PMID:32996998 · one of two readings recorded this

mitochondrial State 3 (ADP-stimulated) oxygen consumption, oxygen electrode

decreases

The IRIC group worsened in State3 when compared to the IR group.

PMID:32996998 · one of two readings recorded this

Mitochondrial State 3 respiration (ADP-stimulated O2 consumption)

decreases

The IRIC group worsened in State3 when compared to the IR group.

PMID:32996998 · one of two readings recorded this

Mitochondrial State 4 respiration (baseline O2 consumption)

IC when associated with ischemia (IRIC) showed difference statistically significant when compared to the SHIC group.

PMID:32996998 · one of two readings recorded this

Respiratory Control Ratio (State3/State4)

There was a statistical difference between the SH group and the IR and IRIC groups (SH x IR; SH x IRIC).

PMID:32996998 · one of two readings recorded this

Serum aspartate aminotransferase (AST)

The serum determination of aspartate aminotransferase (AST) levels noted that the IC has no protective effect on the IRI since there is a significant difference between the groups SHIC x IRIC; SH x IRIC.

PMID:32996998 · one of two readings recorded this

Serum lactate dehydrogenase (LDH)

The serum determination of lactate dehydrogenase (LDH) reveals a gap between SH x IRIC confirming the ineffectiveness of the drug in protecting the hepatocyte.

PMID:32996998 · one of two readings recorded this

Tissue malondialdehyde (MDA), lipid peroxidation marker

no effect

The determination of tissue MDA is a marker of lipid peroxidation and the researchers found no significant difference between groups.

PMID:32996998 · one of two readings recorded this

tissue malondialdehyde (MDA), thiobarbituric acid colorimetric assay

no effect

The determination of tissue MDA is a marker of lipid peroxidation and the researchers found no significant difference between groups.

PMID:32996998 · one of two readings recorded this

Tissue nitrate (NO)

decreases

Regarding the determination of tissue NO, it presented a reduction between IRIC when compared with IR group.

PMID:32996998 · one of two readings recorded this

tissue nitrate (NO3-), chemiluminescence NO/ozone assay

decreases

Regarding the determination of tissue NO, it presented a reduction between IRIC when compared with IR group.

PMID:32996998 · one of two readings recorded this

blood urea nitrogen and plasma nitrate

no effect, no statistically significant differences in urea and nitrate dosages, n = 5

The dosages of urea and nitrate did not present results with statistically significant differences.

PMID:35274519 · one of two readings recorded this

cardiac output (CO) and cardiac output index (COI)

no effect, IC given before C48/80 did not improve CO or COI, n = 5

And IC injection before C48/80 in IC + C48/80 group did not improve either CO or COI (and).

PMID:35274519 · one of two readings recorded this

mean arterial pressure, continuous intra-arterial and non-invasive hemodynamic monitoring in a swine anaphylaxis/vasoplegia model

decreases, hypotension more pronounced when IC given in combination with compound 48/80 than with compound 48/80 alone

Not recorded as a finding: the comparison was against another active treatment

Arterial hypotension was more pronounced in groups receiving C48/80 in association with IC.

PMID:35274519 · one of two readings recorded this

mean arterial pressure (MAP)

decreases, hypotension more pronounced in groups receiving C48/80 combined with IC than C48/80 alone, n = 5

Arterial hypotension was more pronounced in groups receiving C48/80 in association with IC.

PMID:35274519 · one of two readings recorded this

serum creatinine

increases, significant increase in creatinine after 20 minutes in the IC+C48/80 group, n = 5

On the other hand, the creatinine dosage in the IC+C48/80 group showed a significant increase after 20 minutes (, and).

PMID:35274519 · one of two readings recorded this

successful visualization/localization of pulmonary nodule without C-arm assistance

In 2/60 cases, visualization failed.

PMID:36143912 · one of two readings recorded this

hazard of indigo carmine's biodegradation products to human erythrocytes relative to the original dye

Not recorded as a finding: no outcome node for erythrocyte toxicity/hazard; an ex vivo erythrocyte exposure comparing the dye against its degradation products, not an administered-to-human exposure

Toxicity tests indicated that the biodegradation products of indigo carmine are non-toxic to Nicotiana tabacum seeds and are less hazardous to human erythrocytes than the original dye.

PMID:36500632 · one of two readings recorded this

human erythrocyte count / echinocyte formation after indigo carmine exposure

decreases, normal erythrocyte counts significantly decreased after indigo carmine treatment compared with control

Not recorded as a finding: no outcome node for erythrocyte morphology/cytotoxicity assay

The numbers of normal erythrocytes after treating indigo carmine and the concentrations of indigo-carmine degradation products are decreased significantly compared with those of the control sample (p < 0.01) (Table 3).

PMID:36500632 · one of two readings recorded this

human erythrocyte count / morphology (echinocyte formation)

decreases, normal erythrocyte counts significantly decreased after indigo carmine treatment vs control

The numbers of normal erythrocytes after treating indigo carmine and the concentrations of indigo-carmine degradation products are decreased significantly compared with those of the control sample (p < 0.01) (Table 3).

PMID:36500632 · one of two readings recorded this

indigo carmine decolorization rate (%)

increases, decolorization rate increased from 32% to 97% within 2 h with acetosyringone mediator

However, in the presence of the mediator acetosyringone, the decolorization rate of indigo carmine greatly increases from 32% to 97% in 2 h.

PMID:36500632 · one of two readings recorded this

intracellular tyrosinase and lignin peroxidase activity following indigo carmine exposure

increases, significantly increased tyrosinase and lignin peroxidase activity after treatment

Not recorded as a finding: agentless passive sentence; the subject (indigo carmine) is carried from the previous sentence and the sentence does not name it, so it is not self-supporting on its own

Significantly increased intracellular tyrosinase activity and lignin peroxidase activity were also observed (at p < 0.01).

PMID:36500632 · one of two readings recorded this

Nicotiana tabacum seed germination rate

decreases, indigo carmine inhibits seed germination more than control

Indigo carmine inhibits N. tabacum seed germination more than the control (p < 0.05), while the degradation products of indigo carmine have a similar effect on the germination of N. tabacum to that of the control (Table 3).

PMID:36500632 · one of two readings recorded this

spore laccase enzymatic activity

increases, laccase activity significantly higher after indigo carmine treatment vs control strain

The spore laccase activity from strain HL3 after the treatment of indigo carmine was higher significantly than that from control strain (at p < 0.01).

PMID:36500632 · one of two readings recorded this

intervertebral disc cell cytotoxicity (CCK-8 assay)

increases, significant toxicity threshold for indigo carmine at 1 mg/mL, versus 0.5 mg/mL for methylene blue and 2.5 mg/mL for patent blue

Compared with controls, concentrations showing significant toxicity for indigo carmine, methylene blue, and patient blue were 1 mg/mL, 0.5 mg/mL, and 2.5 mg/mL, respectively.

PMID:36804435 · one of two readings recorded this

intervertebral disc cell cytotoxicity of indigo carmine, CCK-8 viability assay, compared with methylene blue and patent blue

increases, significant toxicity threshold for indigo carmine at 1 mg/mL, compared with 0.5 mg/mL for methylene blue and 2.5 mg/mL for patent blue

Not recorded as a finding: this platform holds no node for the endpoint

Compared with controls, concentrations showing significant toxicity for indigo carmine, methylene blue, and patient blue were 1 mg/mL, 0.5 mg/mL, and 2.5 mg/mL, respectively.

PMID:36804435 · one of two readings recorded this

intradiscal staining efficacy (ImageJ quantification)

lower limit of concentration for significant staining efficacy of indigo carmine was 0.25 mg/mL

Lower limits of concentration producing significant staining efficacy of indigo carmine, methylene blue, and patent blue were 0.25 mg/mL, 0.25 mg/mL, and 0.05 mg/mL, respectively.

PMID:36804435 · one of two readings recorded this

adverse events/complications related to IV indigo carmine injection

no effect, no complications related to IV indigo carmine injection

No complication related to IV indigo carmine injection was found.

PMID:38160534 · one of two readings recorded this

intraoperative bladder injury detection rate using indigo carmine

Not recorded as a finding: diagnostic/procedural endpoint about the dye's utility for injury detection (reagent use), not an outcome node representing a bodily effect of the compound

There were 6 (1,4%) bladder injuries during vaginal hysterectomies and 1 (1,1%) bladder injury during vaginal prolapse surgery.

PMID:38160534 · one of two readings recorded this

intraoperative bladder injury detection rate

There were 6 (1,4%) bladder injuries during vaginal hysterectomies and 1 (1,1%) bladder injury during vaginal prolapse surgery.

PMID:38160534 · one of two readings recorded this

E. coli culture viability protection against T4 phage infection

no effect, no significant difference in viable E. coli count between IC-only and IC+T4 groups

Not recorded as a finding: no outcome node for bacterial culture protection/viability assay

There was no statistically significant difference in the number of viable E. coli cells between samples containing IC and IC along with T4 (P > 0.05).

PMID:39862274 · one of two readings recorded this

E. coli culture viability with T4 phage challenge

no effect, no significant difference in viable E. coli between IC-only and IC+T4 groups

There was no statistically significant difference in the number of viable E. coli cells between samples containing IC and IC along with T4 (P > 0.05).

PMID:39862274 · one of two readings recorded this

GFP protein expression in E. coli

no effect, IC did not impact GFP overexpression

It was observed that the addition of IC to an E. coli culture did not impact the overexpression of the example protein (GFP), as the overall production of GFP was comparable to that of the control (without IC).

PMID:39862274 · one of two readings recorded this

MS2 (RNA) bacteriophage titer response to indigo carmine

no effect, indigo carmine ineffective against MS2, unlike the DNA phages tested

Not recorded as a finding: no outcome node exists for bacteriophage titer/antiphage activity; not a human or animal health outcome

Surprisingly, indigo carmine was ineffective against MS2 phages.

PMID:39862274 · one of two readings recorded this

MS2 (RNA) bacteriophage titer response

no effect, IC ineffective against MS2

Surprisingly, indigo carmine was ineffective against MS2 phages.

PMID:39862274 · one of two readings recorded this

selective inactivation of DNA bacteriophages (EC50) by indigo carmine in industrial bioprocess cultures

increases, EC50 values ranging from 0.105 to 0.006 mg/mL

Not recorded as a finding: no matching outcome node - the outcome list covers human/animal clinical and physiological endpoints, not bacteriophage inactivation in an industrial bioprocess

IC demonstrated selective inactivation of DNA phages (P001, T4, T1, T7, λ) with the EC50 values ranging from 0.105 to 0.006 mg/mL while showing no activity against the RNA phage MS2.

PMID:39862274 · one of two readings recorded this

T4 bacteriophage titer reduction

decreases, complete inactivation (>6 log reduction) after overnight incubation at 50°C with 2 mg/mL IC

At 50 °C, IC (2 mg/mL) caused a 2 log decrease after around 12 h and complete inactivation (the titer dropped below the detection limit, i.e., more than 6 log) after overnight incubation (versus around 1 log at 37 °C).

PMID:39862274 · one of two readings recorded this

BLG amyloid-like fibrillation (Far-UV CD structural shift)

increases, shift from native β-sheet to cross-β amyloid architecture

Far-UV CD confirmed a shift from native β-sheet structure to cross-β architecture characteristic of amyloid fibrils.

PMID:42348977 · one of two readings recorded this

BLG protein band intensity by SDS-PAGE after indigo carmine exposure

decreases, disappearance of BLG protein bands at IC concentrations of 0.1 mM or higher, indicating extensive aggregation

Not recorded as a finding: no outcome node for electrophoretic protein aggregation marker

SDS-PAGE showed the disappearance of protein bands at ≥0.1 mM IC, indicating extensive aggregation.

PMID:42348977 · one of two readings recorded this

BLG protein band intensity by SDS-PAGE

decreases, disappearance of protein bands at IC concentrations of 0.1 mM or more

SDS-PAGE showed the disappearance of protein bands at ≥0.1 mM IC, indicating extensive aggregation.

PMID:42348977 · one of two readings recorded this